An Improved 2-step Liquid Culture System for Efficient In Vitro Shoot Proliferation of Sundew (Drosera rotundifolia L.)
نویسنده
چکیده
An efficient procedure for in vitro shoot production of the medicinal plant sundew (Drosera rotundifolia L.) was developed. Of three cytokinins tested, zeatin (ZEA) at a concentration of 2 μM resulted in the formation of large numbers of adventitious shoots on leaf explants. A larger size of the small shoots was achieved by combining a 2-weeks preculture with ZEA (step 1: shoot induction) with a 5-weeks main culture without plant growth regulators (step 2: shoot elongation). Liquid media were superior to semisolid media: An average of 27.4 shoots per leaf explant, and 53.3 shoots per shoot explant were achieved. The 2-step culture system with liquid media permits a comparatively cheap micropropagation of sundew as well as in vitro biomass production, with potential for scale-up.
منابع مشابه
Optimalisation of expression conditions for production of round-leaf sundew chitinase (Drosera rotundifolia L.) in three E. coli expression strains Optimalizácia podmienok expresie droserovej chitinázy (Drosera rotundifolia L.) v troch expresných kmeňoch E. coli
Round-leaf sundew (Drosera rotundifolia L.), family Droseraceae, genus Drosera, is one of a few plant species with a strong antifungal potential. Chitinases of carnivorous plants play an important role in decomposition of chitin-containing cell structures of insect prey. The cell wall of many phytopathogenic fungi also contains chitin, which can be utilized by chitinases, thus round-leaf sundew...
متن کاملEffect of Increased Atmospheric Nitrogen Deposition and Elevated CO2 on Traits Responsible for Carnivory in the Sundews Drosera rotundifolia and Drosera intermedia Senior Honors Thesis by Chloe White Program
Two experiments were conducted to study the effects of increased atmospheric nitrogen deposition and elevated CO2 on investment in carnivory by two sundew species, Drosera rotundifolia and D. intermedia. A field study was conducted with D. rotundifolia at Inverness Mud Lake Bog in Inverness Twp., MI where plants were exposed to simulated future increases in atmospheric nitrogen deposition predi...
متن کاملPhloroglucinol and Silver Nitrate Enhances Axillary Shoot Proliferation in Nodal Explants of Vitex negundo L. âan Aromatic Medicinal Plant
A reliable method is described for rapid proliferation of axillary shoots from nodal segment of mature field-grown aromatic and medicinal plant Vitex negundo through in vitro culture. Of different concentrations of N6-benzyladenine (BA) evaluated as supplements to Murahige and Skoog (MS) medium, BA at 1mg/l was effective in enhancing bud break and shoot regeneration. Response of explants was al...
متن کاملEffect of Liquid Media and In Vitro Pre-acclimatization Stage on Shoot Elongation and Acclimatization of Date Palm (Phoenix dactylifera L.) cv. Najda
The effect of stationary liquid media to improve shoots elongation in date palm cv. Najda and an in vitro pre-acclimatization stage to increase the survival ercentage of plantlets in the greenhouse was investigated. Two basal liquid media were tested: full strength and half strength Murashige and Skoog medium (MS). Media were devoid of plant growth regulators (PGRs), containing 0.5 mg/L 6-benzy...
متن کاملImprovement of shoot-tip culture proliferation in banana using PEG6000. Leila Saeedavi, Ali Soleimani* and Mohammad Esmaeil Amiri
In order to improve the shoot proliferation rate of ‘Dwarf Cavendish’ banana shoot tip cultures, polyethylene glycol (PEG6000) was employed at concentrations of 2, 4, and 6% (w/v). The medium without PEG was considered as negative control. An increasing of shoot proliferation was achieved in MS medium containing 2% PEG. Furthermore, application of 2% PEG significantly increased the shoot dry we...
متن کامل